celeste || 24 || english & 中文 || art blog where i post my oc art and sometimes complain
hehe bacterial transformation successful
i shoved a plasmid (a piece of circular DNA) inside my bacteria and forced it to express a red fluorescent protein (original color is on the right, the whitish color)
fungi: just chillin
bacteria: so u have chosen death
nobody asked but im gonna ramble on about my research project
so my lab works on endophytes, which are microbes that live inside plants and help em grow. my particular project is on some strains of Pseudomonas bacteria that i isolated from agave seeds
they glow in under UV light and they're so good!!!
they're highly antifungal (hypothesis: they're fungal pathogens) and they're super helpful to all the plants i've tested them on so far (carrots/coriander/rice/catnip)
right now a friend from another lab and i are collaborating on a project between her catnip and my Pseudomonas to see if my Pseudomonas can induce the catnip to produce more of the essential oil that attracts cats
i'm hoping to get enough data from my other aspects of my own project to have a scientific article completed by this fall/winterzylphide
so my experience working in the lab is only restricted to my specific lab, but in general, biology research labs in the us all tend to work very similarly (huge wall of text under the read more fkjsda)
i chose plant pathology as my track precisely because i literally cannot handle hurting animals. i know mice as model animals is important for biomedical research, but that's a big no from me on that.
and honestly? most of my fellow grad friends are kinda bad at keeping their lab notebooks up to date. the lab notebook in a Real research setting isn't exactly the same as the notebooks you might have to fill out in class. the lab notebook is a legal document (at least here in the us), so you can't take it home or anything. most of the time we record data on a separate piece of paper (i have several random notebooks laying around for this purpose) and later on transfer it into the actual lab notebook.
in undergrad, there's a lot of following instructions, but at a graduate level, you kind of have to come up with your own instructions since you're solving problems no one else has ever solved before. it can get kind of annoying having to come up with novel solutions each time though, since there can be a lot of trial and error (and wasted time) involved dkjfakdsfj
i'd say a lot of people in academia are doing research to help better the world in some way. unfortunately, academia tends to retain egotistical people who value their reputation and publishable results rather than ones who are genuinely interested in passing on their knowledge. by the time grad students get their phds (and a lot of us drop out before then), they're burnt out by how academics (including people in their own department, or even their own advisors) treat them. i'm lucky my advisor is very laid back and doesn't treat us badly, but there are professors out there who are very mean to their students and basically treat them like slave labor.
from what i've read, nepetalactones activate some smell receptors in a cat's nose, so they're more like pheromones. i'm pretty sure they're not hallucinogenic, but then again, it's not like we can ask the cat.
our test run was on multiple plantlets. we don't test on just one plant because you need multiple points of data to make sure you didn't happen get an outlier. i think we did 7 plantlets per plate, with 3 plates per bacteria, totalling to 21 plants. that way we can get a good average and people can't dismiss us by saying "oh you only got those results because you have a small sample size"
if you really are interested in seeing if lab work is for you, i would recommend talking to professors who are doing research you're interested in and seeing if they would be willing to let you help them for a semester. my school let people do that for credits (or for money, if they were in a work-study program) and it's a good way to gauge if lab work is something you're passionate about. i know people who did bench work (actually doing the experiments instead of just reading/writing about them) for a year and decided they hated it, but it's only something they found out after they had that experience.
uh sorry this got very very long, i've been in a research lab for a while now and i have a lot of thoughts skjfksjf
forbidden glowing bacterial soup
oof my uni extended spring break and is kicking pretty much everyone out of the residence halls because of the coronavirus
labs aren't closing but i need to get all my bacteria and fungi in the -80C freezer just in case
im ending my entire academic career
i've been working with these bacteria for 2 years now and i just learned they're not pure cultures because i put em on some media with arsenic on it for shits and giggles since my labmate made some
can i get some Fs in chat for my data
the madlad really just grew Over the divider huh